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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Excitation Laser:
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
COA:
Application Notes:
Additional reported applications include: block binding of PVR ligands CD226 and CD96 to PVR, immunoprecipitation, western blot
CD155, known as poliovirus receptor (PVR) or nectin-like 5, is a 70 kD type I transmembrane glycoprotein. It is a nectin-like molecule belonging to Ig superfamily. CD155 is primarily found on endothelial cells, monocytes, epithelia and central nervous system. CD155 is an adhesion molecule involved in cell-cell and cell-matrix adhesion through interaction with CD226, CD96 (Tactile), nectin-1, -2, and -3 (CD111, CD112, CD113), and vitronectin. It also serves as a receptor for poliovirus and cytomegalovirus.
Other Names:
Poliovirus receptor (PVR), nectin-like 5
Structure:
Type I glycoprotein, Ig superfamily
Distribution:
Monocytes, endothelial cells, epithelia, central nervous system
Function:
Cell-cell and cell-matrix adhesion
Ligand Receptor:
CD226, CD96, nectin-1, -2, and -3, vitronectin, CD155, poliovirus
Antigen References:
1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Bottino PD, et al. 2005. Mol. Immunol. 42:463 3. Tahara-Hanaoka S, et al. 2004. Intl. Immunol. 16:533
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD155 (PVR)
Human histiocytic lymphoma cells U-937 stained with SKII.4 PE
Purified anti-human CD155 (PVR)
Human histiocytic lymphoma cells U-937 stained with purified SKII.4, followed by anti-mouse IgG FITC
