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PE anti-human CD152 (CTLA-4) Antibody
PE anti-human CD152 (CTLA-4) Antibody
349905 25 tests $95.00     
349906 100 tests $230.00     
Product Details
Clone: L3D10
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.
Application
References:

1. May K, et al. 2005. Blood 105:1114. (Block)
2. Lute K, et al. 2005. Blood 106:3127. (Block)
PHA-stimulated human peripheral blood mononuclear
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with L3D10 PE and CD3 APC

PHA-stimulated human peripheral blood mononuclear
PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control


Compare all formats



Description:

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. Regulatory T cells express high level of CTLA-4. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.
Other Names: Cytotoxic T-Lymphocyte Antigen 4
Structure: Ig superfamily, 33 kD
Distribution: Activated T and B cells
Function: Negative regulator of T cell activation
Ligand Receptor: B7-1 (CD80), B7-2 (CD86)
Antigen
References:

1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.
GeneID: 1493
Latest Publications: View the latest CD152 articles on HighwirePress.com
UniProt: View information about CD152 on UniProt.org
Keywords: PE anti-human CD152 (CTLA-4), L3D10, PE, Cytotoxic T-Lymphocyte Antigen 4, Human , Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human CD152 (CTLA-4)
    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with purified L3D10 conjugated with PE and CD3 APC

    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control

  • PE anti-human CD152 (CTLA-4)
    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with L3D10 PE and CD3 APC

    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control

  • LEAF™ Purified anti-human CD152 (CTLA-4)
    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with purified L3D10 conjugated with PE and CD3 APC

    PHA-stimulated human peripheral blood mononuclear

    PHA-stimulated human peripheral blood mononuclear cells (day-3) stained with CD3 APC and mouse IgG1, κ PE isotype control

  • APC anti-human CD152 (CTLA-4)
    PHA-stimulated (3-day) human peripheral blood

    PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD3 FITC and CD152 (clone L3D10) APC (top) or mouse IgG1 APC isotype control (bottom).





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