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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional reported applications (for the relevant formats) include: Western Blot1,2, immunofluorescencemicroscopy1,3, immunoprecipitation1,4, block angiogenesis in vitro4,5, inhibit VE-cadherin reorganization4, and induce endothelial cell apoptosis4.
CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of 5 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.
VE-Cadherin, cadherin-5, CDH5
A member of the cadherin family, is a calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of 5 extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with purified BV9 conjugated with PE
PE anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with BV9 PE
APC anti-human CD144 (VE-Cadherin)
Human umbilical vein endothelial cells, HUVEC, stained with BV9 APC