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PE Anti-human CD141 (Thrombomodulin) Antibody
Material Safety Data Sheet (MSDS) PE Anti-human CD141 Thrombomodulin Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
PE Anti-human CD141 (Thrombomodulin) Antibody
344103 25 tests $125.00     
344104 100 tests $250.00     
Clone: M80
Isotype: Mouse IgG1, κ
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Human peripheral blood monocytes stained

Human peripheral blood monocytes stained with M80 PE
LPS-stimulated (overnight) human peripheral blood

LPS-stimulated (overnight) human peripheral blood mononuclear cells stained with M80 PE (gated on CD14+ cells)


Description:

CD141 is a 75KDa, single chain, type I membrane glycoprotein; is also known as thrombomodulin, TM, THRM, THBD or fetomodulin. CD141 is an important cofactor in the protein C anticoagulant system. After binding to its ligand thrombin, CD141 activates protein C, which degrades clotting factors Va and VIIIa and as a consequence, the amount of thrombin is reduced. CD141 is expressed on macrophages, monocytes, vascular endothelial cells and keratinocytes. Besides anti-coagulation function, CD141 is also involved in embryonic and atherosclerotic plaque development.

Other Names: Thrombomodulin, TM, THRM, THBD, Fetomodulin, BDCA-3
Structure: Single chain, type I membrane glycoprotein, 75KDa
Distribution: Macrophages, monocytes, vascular endothelial cells, keratinocytes
Function: After thrombin binding, CD141 activates protein C, which degrades clotting factors Va and VIIIa, and reduces the amount of thrombin generated
Ligand Receptor: Thrombin
Interaction: Protein C, Thrombin-activatable fibrinolysis inhibitor (TAFI), Platelet factor 4 (PF4)
Antigen
References:
1. Suzuki, K., et al. 1987. EMBO J. 6: 1891
2. Esmon, C.T., et al., 1989. J Biol Chem. 264: 4743
3. Delvaeye, M., et al. 2009. N Engl J Med. 361: 345
4. Shi, C.S., et al. 2008. Blood.112: 3661
5. Chen, L.C., et al. 2009. J Infect. 58: 368
PE Mouse IgG1, κ Isotype CtrlMOPC-21FC,ICFC
Cell Staining Buffer (FBS)FC,ICC,ICFC
RBC Lysis Buffer (10X)FC,ICFC