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PE anti-human CD14 Antibody
PE anti-human CD14 Antibody
325605 25 tests $95.00     
325606 100 tests $210.00     
Product Details
Clone: HCD14
Isotype: Mouse IgG1, κ
Isotype Control:PE Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human Cross-Reactivity: Horse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and immunofluorescence microscopy.
Application
References:

1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood monocytes stained
Human peripheral blood monocytes stained with HCD14 PE


Compare all formats



Description:

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
Other Names: LPS receptor
Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: Monocytes, macrophages, granulocytes (low)
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor: LPS
Antigen
References:

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
GeneID: 929
Latest Publications: View the latest CD14 articles on HighwirePress.com
UniProt: View information about CD14 on UniProt.org
Keywords: PE anti-human CD14, HCD14, PE, LPS receptor, Human Cross-Reactivity: Horse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with purified HCD14, followed by anti-mouse IgG FITC

  • FITC anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 FITC

  • PE anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 PE

  • APC anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 APC

  • Alexa Fluor® 488 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 alexa Fluor® 647

  • Alexa Fluor® 700 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 Alexa Flour® 700

  • Pacific Blue™ anti-human CD14
    Human peripheral blood monocytes were

    Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).

  • PE/Cy7 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 PE/Cy7

  • APC/Cy7 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 APC/Cy7

  • PerCP/Cy5.5 anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with HCD14 PerCP/Cy5.5

  • Biotin anti-human CD14
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with biotinylated HCD14 and then detected with Sav-PE

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