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The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
6H6 antibody does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2.
Application References:
1. Sun Q, et al. 1996. Blood 87:83. (IP, WB) 2. Herling M, et al. 2003. Blood 101:5007. (IHC) 3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.
Other Names:
IL-3Rα, IL-3 Receptor alpha
Structure:
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
PE anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
Purified anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
PE/Cy5 anti-human CD123
Human peripheral blood lymphocytes stained with CD45RO FITC and 6H6 PE/Cy5
PE/Cy7 anti-human CD123
Human peripheral blood lymphocytes stained with CD45RO FITC and 6H6 PE/Cy7
APC anti-human CD123
CD14- human peripheral blood mononuclear cells stained with ILT7 PE and 6H6 APC
FITC anti-human CD123
Human peripheral blood leukocytes stained with CD45RO APC and 6H6 FITC (gated on lymphocyte population)
PerCP/Cy5.5 anti-human CD123
Human peripheral blood lymphocytes stained with 6H6 PerCP/Cy5.5 and UCHL1 FITC
Brilliant Violet 421™ anti-human CD123
Human peripheral blood leukocytes were stained with HLA-DR FITC and CD123 (clone 6H6) Brilliant Violet 421™ (top) or mouse IgG1 Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphocyte population.
Brilliant Violet 650™ anti-human CD123
Human peripheral blood leukocytes were stained with HLA-DR FITC and CD123 (clone 6H6) Brilliant Violet 650™ (top) or mouse IgG1 Brilliant Violet 650™ isotype control (bottom).
