The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC-Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications: immunoprecipitation(1), blocking the interaction between ICAM-2 and LFA-1(1), immunohistochemical staining(2) of frozen tissue sections
Application References:
1. DeFougerolles A.R.,et al.1991.J. Exp.Med.174:253. 2. Van Herpen C.ML.,et al.2005.Clin. Cancer Res.11:1899
Human peripheral blood lymphocytes stained with CBR-IC2/2 PE
Description:
CD102 is a 55 kD type I transmembrane glycoprotein, belongs to immunoglobulin superfamily, also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and in costimulation of immune response. CD102 is a counter receptor of LFA-1 (CD11a/CD18), DC-SIGN and MAC-1.
Other Names:
Intercellular Adhesion Molecule-2, ICAM-2
Structure:
55 kD type I transmembrane glycoprotein, Ig superfamily