The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications: immunoprecipitation(1), blocking the interaction between ICAM-2 and LFA-1(1), immunohistochemical staining(2) of frozen tissue sections
Application References:
1. DeFougerolles A.R.,et al.1991.J. Exp.Med.174:253. 2. Van Herpen C.ML.,et al.2005.Clin. Cancer Res.11:1899
Human peripheral blood lymphocytes stained with CBR-IC2/2 PE
Description:
CD102 is a 55 kD type I transmembrane glycoprotein, belongs to immunoglobulin superfamily, also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and in costimulation of immune response. CD102 is a counter receptor of LFA-1 (CD11a/CD18), DC-SIGN and MAC-1.
Other Names:
Intercellular Adhesion Molecule-2, ICAM-2
Structure:
55 kD type I transmembrane glycoprotein, Ig superfamily
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