The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: T cell activation.
Application References:
1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC) 2. Joseph, A., et al. 2008. J. Virol. 82:3078. (FC) PubMed
Human peripheral blood lymphocytes stained with IP26 PE
Description:
The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.
Other Names:
α/β TCR, TCR α/β
Structure:
Ig superfamily, with CD3 forms CD3/TCR complex
Distribution:
T cells, thymocytes
Function:
Antigen recognition, T cell activation
Ligand Receptor:
Peptide bound to MHC
Antigen References:
1. Marchalonis, J., et al., 2002. J. Mol. Recognit. 15:260.
