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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The H57-597 antibody does not cross-react with γ/δ TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate α/β TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 109214).
Application References:
1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296. 2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP) 3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim) 4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) 5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete) 6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete) 7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC) 8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC) 9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC) 10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed 11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
C57BL/6 splenocytes stained with H57-597 Pacific Blue™
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
Other Names:
TCR-β chain, TCR-β, β-TCR
Structure:
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution:
Majority of T cells and thymocytes (correlated to differentiation)
Function:
Antigen recognition, T cell activation
Ligand Receptor:
Peptide bound-MHC class I and II
Antigen References:
1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523. 2. Huppa JB, et al. 2003. Nat. Immunol. 4:749. 3. Kubo R, et al. 1989. J. Immunol. 142:2736.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 APC
Biotin anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with biotinylated H57-597, followed by Sav-PE
FITC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 FITC
LEAF™ Purified anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained
with H57-597 PE and TCRγ/δ (UC7-
13D5) FITC
PE anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained
with H57-597 PE and TCRγ/δ (UC7-
13D5) FITC
PE/Cy5 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cy5.
Purified anti-mouse TCR β chain
C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Alexa Fluor® 647
PE/Cy7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cy7
Alexa Fluor® 700 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 700
Pacific Blue™ anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Pacific Blue™
Brilliant Violet 421™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 421™ (filled histrogram) or Armenian hamster IgG Brilliant Violet 421™ isotype control (open histogram).
PerCP/Cy5.5 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 PerCP/Cy5.5
Brilliant Violet 570™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) Brilliant Violet 570™ (filled histogram) or Armenian hamster IgG Brilliant Violet 570™ isotype control (open histogram).
