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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102210).
Application References:
1. Noto K, et al. 1995. Int. Immunol. 7:835. 2. Ridger VC, et al. 2001. J. Immunol. 166:3484. 3. Jia W, et al. 2005. Blood 106:3854.PubMed 4. Economopoulou M, et al. 2005. Blood 106:3831. 5. Lawson BR, et al. 2007. J. Immunol. 178:5366. 6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed 7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed 8. Kim DT, et al. 2008. Blood 111:2929.PubMed 9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed 10. Carlson TR, et al. 2008. Development. 135:2193. PubMed 11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed 12. Li G, et al. 2011. J. Mol Cell Biol. PubMed.
C57BL/6 mouse bone marrow cells stained with HM?1-1 Pacific Blue™
CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse / rat CD29
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
C57BL/6 mouse splenocytes stained with HMβ1-1 PE
Purified anti-mouse / rat CD29
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
C57BL/6 mouse splenocytes stained with HMβ1-1 PE
FITC anti-mouse / rat CD29
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
C57BL/6 mouse splenocytes stained with HMβ1-1 PE
PE anti-mouse / rat CD29
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
C57BL/6 mouse splenocytes stained with HMβ1-1 PE
LEAF™ Purified anti-mouse / rat CD29
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
C57BL/6 mouse splenocytes stained with HMβ1-1 PE
Alexa Fluor® 488 anti-mouse / rat CD29
C57BL/6 mouse splenocytes stained with HMβ1-1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse / rat CD29
C57BL/6 mouse splenocytes stained with HMβ1-1 Alexa Fluor® 647
APC anti-mouse / rat CD29
C57BL/6 mouse splenocytes stained with HMβ1-1 APC
Alexa Fluor® 700 anti-mouse / rat CD29
C57BL/6 mouse bone marrow cells stained with HM?1-1 Alexa Fluor® 700
PE/Cy5 anti-mouse / rat CD29
C57BL/6 mouse splenocytes stained with HMβ1-1 PE/Cy5
PE/Cy7 anti-mouse / rat CD29
C57BL/6 mouse splenocytes stained with HMβ1-1 PE/Cy7
Pacific Blue™ anti-mouse / rat CD29
C57BL/6 mouse bone marrow cells stained with HM?1-1 Pacific Blue™
APC/Cy7 anti-mouse/rat CD29
C57BL/6 bone marrow cells stained with HMb1-1 APC/Cy7
