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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 108712).
Application References:
1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP) 2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP) 3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt) 4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete) 5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete) 6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662. 7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block) 8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143. 9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete) 10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete) 11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC) 12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed 13. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
C57BL/6 splenocytes stained with PK136 Pacific Blue™
NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
Other Names:
NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Structure:
NKR-P1 gene family
Distribution:
NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function:
NK cell activation, IFN-γ production, cytotoxic granule release
Antigen References:
1. Lanier LL. 1997. Immunity 6:371. 2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613. 3. Koo GC, et al. 1986. J. Immunol. 137:3742. 4. Giorda R, et al. 1991. J. Immunol. 147:1701.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) APC (top) or mouse IgG2a APC isotype control (bottom).
Biotin anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
FITC anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) FITC and NKG2D PE.
LEAF™ Purified anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with DX5 APC and LEAF™ purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), followed by anti-mouse IgG2a FITC.
PE anti-mouse NK-1.1
C57BL/6 splenocytes stained with PK136 PE and CD11c APC
C57BL/6 mouse splenocytes stained with PK136 PE
Purified anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), followed by anti-mouse IgG2a FITC.
PE/Cy7 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cy7.
PE/Cy5 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cy5.
Alexa Fluor® 488 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and NK1.1 (clone PK136) Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE and NK1.1 (clone PK136) Alexa Fluor® 647 (top) or mouse IgG2a Alexa Fluor® 647 isotype control (bottom).
Pacific Blue™ anti-mouse NK-1.1
C57BL/6 splenocytes stained with PK136 Pacific Blue™
APC/Cy7 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b/DX5 FITC and NK1.1 (clone PK136) APC/Cy7 (top) or mouse IgG2a APC/Cy7 isotype control (bottom).
PerCP anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) FITC and NK1.1 (clone PK136) PerCP (top) or mouse IgG2a PerCP isotype control (bottom).
PerCP/Cy5.5 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PerCP/Cy5.5.
Alexa Fluor® 700 anti-mouse NK-1.1
C57BL/6 splenocytes were stained with CD49b (clone DX5) PE and NK1.1 (clone PK136) Alexa Fluor® 700 (top) or mouse IgG2a Alexa Fluor® 700 isotype control (bottom).
Brilliant Violet 421™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b/DX5 PE and NK1.1 (clone PK136) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b/DX5 FITC and NK1.1 (clone PK136) Brilliant Violet 570™ (top) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
