The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
ELISA Detection1-3 or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. No. 503702/503704) as capture antibody and recombinant mouse IL-2 (Cat. No. 563001) as the standard. Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization1,7: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-2 bioactivity in vivo and in vitro (Cat. No. 503812). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining2 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, In Vivo Capture7, and immunocytochemistry. Note: For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431001 to 431006) are specially developed and recommended.
Application References:
1. Abrams, J., et al., 1992. Immunol. Rev. 127: 5. 2. Sander, B., et al., 1993. J. Immunol. Meth. 166: 201. 3. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. 4. Klinman, D., et al., 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. 5. Mo, X., et al., 1995. J. Virol. 69: 1288. 6. Karulin, A., et al., 2000. J. Immunol. 164: 1862. 7. Finkelman, F., et al., 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. 8. Ko, S-Y., et al., 2005. J. Immunol. 175:3309. 9. Kang, S. S. and Allen, P. M., 2005. J. Immunol. 174:5382. 10. Lawson, B. R., et al., 2007. J. Immunol. 178:5366.
PMA+ionomycin-stimulated C57BL/6 mouse splenocytes (6 hours) intracellular stained with with CD3 (17A2) PE and JES6-5H4 Pacific Blue™
Description:
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2). This is a neutralizing antibody.
Other Names:
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Structure:
Cytokine; 15-30 kD (Mammalian)
Regulation:
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Cellular Sources:
T cells
Cellular Targets:
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors:
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Bioactivity/Activities:
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Taniguchi, T., et al., 1993. Cell 73:5. 3. Nistico, G. 1993. Prog. Neurobiol. 40:463. 4. Waldmann, T., et al., 1993. Ann. NY Acad. Sci. 685:603.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
