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The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).
Application References:
1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) 2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) 3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) 4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) 5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) 6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) 7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP) 8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™
The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).
Other Names:
MHC class II
Structure:
MHC class II
Distribution:
B and activated T cells, APCs of H-2b mice
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD4
Antigen References:
1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
Pacific Blue™ anti-mouse I-Ab, AF6-120.1, Pacific Blue™, MHC class II, Mouse b haplotype, cross-reacts with H-2k and H-2u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s)., Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with biotinylated AF6-120.1, followed by Sav-PE
FITC anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 FITC
PE anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 PE
Purified anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with purified AF6-120.1, followed by anti-mouse IgG2a FITC
Alexa Fluor® 488 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 PerCP/Cy5.5
APC anti-mouse I-Ab
C57BL/6 mouse splenocytes stained with AF6-120.1 APC
PE/Cy7 anti-mouse I-Ab
C57BL/6 splenocytes stained with AF6-120.1 PE/Cy7
Pacific Blue™ anti-mouse I-Ab
C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™
