Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, Western blotting2, and immunohistochemical staining of acetone-fixed frozen sections.
The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the α3 domain of Kd.
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Kd haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323. 3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Pacific Blue™ anti-mouse H-2Kd, SF1-1.1, Pacific Blue™, PB, MHC class I, Mouse d haplotype, Weakly cross-reacts with H-2k haplotype, Does not cross react with other haplotypes (e.g., b, j, p, q, s, v)., Flow Cytometry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse H-2Kd
Balb/c mouse splenocytes stained with biotinylated SF1-1.1, followed by Sav-PE
FITC anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 FITC
PE anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 PE
Purified anti-mouse H-2Kd
Balb/c mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
C57BL/6 mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
Alexa Fluor® 488 anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse H-2Kd
BALB/c mouse splenocytes stained with SF1-1.1 Alexa Fluor® 647
Pacific Blue™ anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Pacific Blue™
C57BL/6 mouse splenocytes stained with SF1-1.1 Pacific Blue™
PerCP/Cy5.5 anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 PerCP/Cy5.5
