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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The GL1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105010).
Application References:
1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP) 2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC) 3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) 4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block) 5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block) 6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) 7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) 8. Lawson BR, et al. 2007. J. Immunol. 178:5366. 9. Turnquist HR, et al. 2007. J. Immunol. 178:7018. 10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Other Names:
B7-2, B70, Ly-58
Structure:
Ig superfamily, 80 kD
Distribution:
B and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function:
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD86
C57BL/6 mouse splenocytes stained with biotinylated GL-1,followed by Sav-PE
FITC anti-mouse CD86
LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 FITC
LEAF™ Purified anti-mouse CD86
C57BL/6 mouse splenocytes stained with LEAF™ purified GL-1,followed by anti-rat IgG FITC
PE anti-mouse CD86
LPS (3-day) stimulated BALB/c mouse splenocytes stained with GL-1 PE
Purified anti-mouse CD86
C57BL/6 mouse splenocytes stained with purified GL-1,followed by anti-rat IgG FITC
APC anti-mouse CD86
LPS-stimulated (3 days) C57BL/6 mouse splenocytes stained with GL-1 APC
PE/Cy7 anti-mouse CD86
C57BL/6 mouse splenocytes stained with GL-1 PE/Cy7
