The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC7
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining2,6 of acetone-fixed frozen sections, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105010).
Application References:
1. Hathcock, K.S., et al., 1993. Science 262:905. 2. Inaba, K., M., et al., 1994. J. Exp. Med. 180:1849. 3. Hathcock, K.S., et al., 1994. J. Exp. Med. 180:631. 4. Krummel, M.F., et al., 1995. J. Exp. Med. 182:459. 5. Liu, Y., et al., 1997. J. Exp. Med. 185:251. 6. Herold, K.C., et al., 1997. J. Immunol. 158:984. 7. Shih, F. F., et al., 2006. J. Immunol. 176:3438. 8. Lawson, B. R., et al., 2007. J. Immunol. 178:5366. 9. Turnquist, H.R., et al., 2007. J. Immunol. 178:7018.
CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The GL1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1).
Other Names:
B7-2, B70, Ly-58
Structure:
Ig superfamily, 80 kD
Distribution:
B and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function:
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Hathcock, K.S., et al., 1993. Science 262:905. 3. Freeman, G.J., et al., 1993. Science 262:907. 4. Carreno, B.M., et al., 2002. Annu. Rev. Immunol. 20:29.
