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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104416).
Application References:
1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block) 2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block) 3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block) 4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (C') 5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461. 6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC) 7. Olver S, et al. 2006. Cancer Res. 66:571. 8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed 9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed 10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed 11. Lee JW, et al. 2006. Nature Immunol. 8:181. 12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed 13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Pacific Blue™ (top) or rat IgG2a Pacific Blue™ isotype control (bottom).
CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1.
Other Names:
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Structure:
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution:
Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function:
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand Receptor:
CD34, GlyCAM-1, MAdCAM-1
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC (filled histogram) or rat IgG2a FITC isotype control (open histogram).
Biotin anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with biotinylated CD62L (clone MEL-14) (filled histogram) or rat IgG2a isotype control (open histogram), followed by Sav-PE.
FITC anti-mouse CD62L
C57BL/6 bone marrow cells stained with MEL-14 FITC
LEAF™ Purified anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD62L (clone MEL-14) (filled histogram) or rat IgG2a isotype control (open histogram), followed by anti-rat IgG FITC.
PE anti-mouse CD62L
C57BL/6 mouse splenocytes stained with MEL-14 PE
PE/Cy5 anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) PE/Cy5 (filled histogram) or rat IgG2a PE/Cy5 isotype control (open histogram) (gated on total cell population).
Purified anti-mouse CD62L
C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
PE/Cy7 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) PE/Cy7 (filled histogram) or rat IgG2a PE/Cy7 isotype control (open histogram).
Alexa Fluor® 488 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 647 and CD62L (clone MEL-14) Alexa Fluor® 488 (top) or rat IgG2a Alexa Fluor® 488 isotype control (bottom).
Alexa Fluor® 647 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom).
Pacific Blue™ anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD3 Alexa Fluor® 488 and CD62L (clone MEL-14) Pacific Blue™ (top) or rat IgG2a Pacific Blue™ isotype control (bottom).
Alexa Fluor® 700 anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) Alexa Fluor® 700 (filled histogram) or rat IgG2a Alexa Fluor® 700 isotype control (open histogram) (gated on total cell population).
APC/Cy7 anti-mouse CD62L
C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC/Cy7 (red histogram) or rat IgG2a FITC isotype control (blue histogram).
PerCP/Cy5.5 anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) PerCP/Cy5.5 (filled histogram) or rat IgG2a PerCP/Cy5.5 isotype control (open histogram) (gated on myeloid cell population).
PerCP anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) PerCP (filled histogram) or rat IgG2a PerCP isotype control (open histogram) (gated on myeloid cell population).
Brilliant Violet 421™ anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on total cell population.
Brilliant Violet 570™ anti-mouse CD62L
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on total cell population.
Brilliant Violet 605™ anti-mouse CD62L
C57BL/6 mouse splenocytes cells were stained with CD62L (clone MEL-14) Brilliant Violet 605™.
