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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMα2 antibody.
DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2 integrin, VLA-2 α chain, and integrin α2 chain. CD49b non-covalently associates with CD29 (β1 integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.
1. Arase H, et al. 2001. J. Immunol. 167:1141. 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 3. Sasaki K, et al. 2003. Int. Immunol. 15:701. 4. Inoue O, et al. 2003. J. Cell Biol. 160:769.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with NK1.1 PE and DX5 APC
Biotin anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with biotinylated DX5, followed by Sav-PE
FITC anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with NK1.1 PE and DX5 FITC
PE anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with NK1.1 (PK136) FITC and DX5 PE
Purified anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with DX5 purified, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with NK1.1 PE and DX5 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with DX5 Alexa Fluor® 647
PerCP/Cy5.5 anti-mouse CD49b (pan-NK cells)
C57BL/6 splenocytes stained with DX5 PerCP/Cy5.5
Pacific Blue™ anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes stained with NK1.1 (PK136) PE and DX5 Pacific Blue™
APC/Cy7 anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and CD49b (clone DX5) APC/Cy7.
PE/Cy7 anti-mouse CD49b (pan-NK cells)
C57BL/6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and CD49b (clone DX5) PE/Cy7.
