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The CD45.1 antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The CD45.1 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This CD45.1 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy9.
Application References:
1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) 2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) 3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP) 4. Suzuki K, et al. 2000. Immunity 13:691. (IHC) 5. Werner N, et al. 2002. Arterioscler Thromb Vasc Biol. 22:1567. (IHC) 6. Lessner SM, et al. 2002 Am J Pathol. 160:2145. (FC, IHC) 7. Chen CC, et al. 2005. Proc Natl Acad Sci USA. 102:11408 (IHC) 8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC) 9. Mende I, et al. 2006 Blood 107:1383. (IF, IHC, FC) 10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC) 11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed 12. Pascal V, et al.2007.J. Immunol. 179:1751. PubMed 13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed 14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed 15. BeamerCA, et al.2007. Am J Respir Cell Mol Biol. 37:729. (FC) PubMed 16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed 17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed 18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
SJL mouse splenocytes stained with A20 Pacific Blue™
CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names:
T200, Ly-5.1, LCA
Structure:
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution:
All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice
Function:
Phosphatase, T and B cell activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD45.1
SJL mouse splenocytes stained with biotinylated A20, followed by Sav-PE
FITC anti-mouse CD45.1
SJL mouse splenocytes stained with A20 FITC
PE anti-mouse CD45.1
SJL (solid line) and C57BL/6 (broken
line) splenocytes stained with
A20 PE
Purified anti-mouse CD45.1
SJL mouse splenocytes stained with purified A20, followed by anti-mouse IgG2a FITC
APC anti-mouse CD45.1
SJL mouse splenocytes stained with A20 APC
APC/Cy7 anti-mouse CD45.1
SJL (solid line) and C57BL/6 (broken
line) splenocytes stained with
A20 PE
Alexa Fluor® 488 anti-mouse CD45.1
SJL mouse splenocytes stained with A20 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD45.1
SJL mouse splenocytes stained with A20 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD45.1
SJL mouse splenocytes stained with A20 Pacific Blue™
Alexa Fluor® 700 anti-mouse CD45.1
SJL mouse splenocytes stained with A20 Alexa Fluor® 700
PerCP anti-mouse CD45.1
SJL mouse splenocytes stained with A20 PerCP
PerCP/Cy5.5 anti-mouse CD45.1
SJL mouse splenocytes stained with A20 PerCP/Cy5.5
PE/Cy7 anti-mouse CD45.1
SJL splenocytes stained with A20 PE/Cy7
Brilliant Violet 421™ anti-mouse CD45.1
SJL mouse splenocytes were stained with CD45.1 (clone A20) Brilliant Violet 421™ (filled histogram) or mouse IgG2a Brilliant Violet 421™ isotype control (open histogram).
C57BL/6 mouse splenocytes were stained with CD45.1 (clone A20) Brilliant Violet 421™ (filled histogram) or mouse IgG2a Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-mouse CD45.1
SJL mouse splenocytes were stained with CD45.1 (clone A20) Brilliant Violet 570™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (open histogram).
