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The CD45 antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions. The solution is free of unconjugated Pacific Blue?.
Concentration:
0.5 mg/ml
Storage & Handling:
The CD45 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This CD45 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103120).
Application References:
1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, C') PubMed 2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) 3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) 4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC) 5. Seaman WE. 1983. J. Immunol. 130:1713. (C') 6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC) 7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed 8. Choudhary SK, et al. 2012. J. Virol. 86:114. PubMed.
C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Other Names:
T200, Ly-5, LCA
Structure:
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution:
All hematopoietic cells except mature erythrocytes and platelets
Function:
Phosphatase, T and B cell activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 APC
Biotin anti-mouse CD45
C57BL/6 mouse splenocytes stained with biotinylated 30-F11, followed by Sav-PE
FITC anti-mouse CD45
C57BL/6 splenocytes stained with 30-F11 FITC
PE anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 PE
PE/Cy5 anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 PE/Cy5
Purified anti-mouse CD45
BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
PE/Cy7 anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 PE/CY7
APC/Cy7 anti-mouse CD45
C57BL/6 splenocytes stained with 30-F11 FITC
LEAF™ Purified anti-mouse CD45
C57BL/6 mouse splenocytes stained with LEAF™ purified 30-F11, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD45
C57BL/6 mouse splenocytes stained with Pacific Blue™ 30-F11
Alexa Fluor® 700 anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 700
PerCP/Cy5.5 anti-mouse CD45
C57BL/6 splenocytes stained with 30-F11 PerCP/Cy5.5
PerCP anti-mouse CD45
C57BL/6 mouse splenocytes stained with 30-F11 PerCP
Brilliant Violet 421™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 421™ (filled histogram) or rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Brilliant Violet 570™ (filled histogram) or rat IgG2b Brilliant Violet 570™ isotype control (open histogram).
