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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletions. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletions8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ) 2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ) 3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1. 4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB) 5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF) 6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (C') 7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ) 8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete) 9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete) 10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete) 11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block) 12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ) 13. Ko SY, et al. 2005. J. Immunol. 175:3309. 14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC) 15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC) 16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ) 17. Xiao S, et al. 2007. J. Exp. Med. 204:1691. 18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed. 19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed 20. Guo Y, et al. 2008. Blood 112:480. PubMed 21. Kenna TJ, et al. 2008. Blood 111:2091. 22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed 23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed 24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed 25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed 26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed 27. Qi Q, et al. 2009. Blood 114:564. PubMed
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) Pacific Blue™ or Armenian hamster IgG Pacific Blue™ isotype control.
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Other Names:
CD3ε, T3, CD3
Structure:
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
TCR signal transduction, T cell activation, antigen recognition
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
Biotin anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with biotinylated CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by Sav-PE.
FITC anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
LEAF™ Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.
PE anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE and CD19 FITC.
PE/Cy5 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE/Cy5 (filled histogram) or Armenian hamster IgG PE/Cy5 isotype control (open histogram).
Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.
PE/Cy7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PE/Cy7 (filled histogram) or Armenian hamster IgG PE/Cy7 isotype control (open histogram).
Alexa Fluor® 488 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) Alexa Fluor® 488 (filled histogram) or Armenian hamster IgG Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) Alexa Fluor® 647 (filled histogram) or Armenian hamster IgG Alexa Fluor® 647 isotype control (open histogram).
PerCP anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PerCP (filled histogram) or Armenian hamster IgG PerCP isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) PerCP/Cy5.5 (filled histogram) or Armenian hamster IgG PerCP/Cy5.5 isotype control (open histogram).
APC/Cy7 anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC/Cy7 (filled histogram) or Armenian hamster IgG APC/Cy7 isotype control (open histogram).
Pacific Blue™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) Pacific Blue™ or Armenian hamster IgG Pacific Blue™ isotype control.
Brilliant Violet 421™ anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD3ε (clone 145-2C11) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
Ultra-LEAF™ Purified anti-mouse CD3ε
C57BL/6 mouse splenocytes were stained with LEAF™ purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.
