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The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections, and induction of B cell proliferation1.
Application References:
1. Oliver AM, et al. 1997. J. Immunol. 158:1108. 2. Howard M, et al. 1993. Science 262:1056.
C57BL/6 mouse splenocytes stained with 90 Pacific Blue™
CD38 is a 42 kD glycoprotein, also known as T10. It is an ADP-ribosyl hydrolase, expressed on B cells, NK cells, subset of T cells, brain, muscle, and kidney. In the mouse, CD38 expression is downregulated on mouse germinal center B cells and plasma cells, whereas this is not the case for humans. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, as well as adhesion and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
Other Names:
T10
Structure:
ADP-ribosyl hydrolase, 42 kD
Distribution:
B cells, NK cells, T cell subset, brain, muscle, kidney
Function:
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand Receptor:
CD31, hyaluronic acid
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Shubinsky G, et al. 1997. Immunity 7:315. 3. Cesano A, et al. 1998. J. Immunol. 160:1106. 4. Cockayne DA, et al. 1998. Blood 92:1324.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 APC
Biotin anti-mouse CD38
C57BL/6 mouse splenocytes stained with biotinylated 90, followed by Sav-PE
FITC anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 FITC
PE anti-mouse CD38
C57BL/6 splenocytes stained with 90 FITC
Purified anti-mouse CD38
C57BL/6 splenocytes stained with purified 90, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 Alexa Fluor? 488
Alexa Fluor® 647 anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 Alexa Fluor? 647
PE/Cy7 anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 PE/Cy7
Pacific Blue™ anti-mouse CD38
C57BL/6 mouse splenocytes stained with 90 Pacific Blue™
