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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102014). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block) 2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block) 3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block) 4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block) 5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete) 6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete) 7. Lei TC, et al. 2005. Blood 105:4865. (Deplete) 8. Pasare C, et al. 2004. Immunity 21:733. (Deplete) 9. León-Ponte M, et al. 2007. Blood 109:3139. 10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete) 11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
Con A-stimulated (3 days) C57BL/6 mouse splenocytes stained with PC61 Pacific Blue™
CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Other Names:
IL-2Rα, Ly-43, p55, Tac
Structure:
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution:
Activated T and B cells, thymocyte subsets, pre-B cells, T regulatory cells
Function:
IL-2 receptor
Ligand Receptor:
IL-2
Antigen References:
1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD25
Con A-stimulated (3 days)splenocytes stained with PC61 APC
Biotin anti-mouse CD25
Con A-stimulated C57BL/6 splenocytes (3 days) stained with PC 61 biotin, followed by Sav-PE
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with LEAF™ purified PC61, followed by anti-rat IgG FITC
PE anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 PE
PE/Cy5 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 PE/Cy5
Purified anti-mouse CD25
C57BL/6 mouse splenocytes stained with purified PC61, followed by anti-rat IgG FITC
PE/Cy7 anti-mouse CD25
Con A-stimulated (2 days) BALB/c mouse splenocytes stained with PC61 PE/Cy7
Alexa Fluor® 488 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with PC61 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD25
Con A-stimulated (3 days) C57BL/6 mouse splenocytes stained with PC61 Pacific Blue™
Alexa Fluor® 700 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with Alexa Fluor® 700
APC/Cy7 anti-mouse CD25
Con A-stimulated (day-3) C57BL/6 mouse splenocytes stained with PC61 APC/Cy7
PerCP/Cy5.5 anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC61 PerCP/Cy5.5
PerCP anti-mouse CD25
Con A-stimulated C57BL/7 splenocytes (Day 3) stained with PC61 PerCP
Brilliant Violet 421™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD25 (clone PC61) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 605™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 PE and CD25 (clone PC61) Brilliant Violet 605™.
Brilliant Violet 650™ anti-mouse CD25
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD25 (clone PC61) Brilliant Violet 650™.
Ultra-LEAF™ Purified anti-mouse CD25
Con A-stimulated (3 days) BALB/c mouse splenocytes stained with LEAF™ purified PC61, followed by anti-rat IgG FITC
