The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC4
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 101810).
Application References:
1. Springer, T., et al., 1978. Eur. J. Immunol. 8:539. 2. Crowley, M., et al., 1989. Cell. Immunol. 118:108. 3. Veillette, A., et al., 1989. J. Exp. Med. 170:1671. 4. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245.
C57BL/6 mouse splenocytes stained with M1/69 Pacific Blue™ M1/69
Description:
CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
Other Names:
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Structure:
GPI-linked sialoglycoprotein, 35-45 kD
Distribution:
B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
Function:
Regulates B cell proliferation and differentiation
Ligand Receptor:
P-selectin, CD24
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Aigner, S., et al., 1997. Blood 89:3385. 3. Hough, M.R., et al., 1996. J. Immunol. 156:479. 4. Liu, Y., et al., 1992. J. Exp. Med. 175:437.
