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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking2-4 of ligand binding, and immunohistochemical staining5 of acetone-fixed frozen sections.
Application References:
1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP) 2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block) 3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block) 4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block) 5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Pacific Blue™ (orange histogram), or rat IgG2a, κ Pacific Blue™(blue histogram).
CD23 is a 45 kD protein, also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion. The B3B4 antibody is useful for blocking IgE activity in vivo.
Other Names:
FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
Structure:
C-lectin family, type II transmembrane glycoprotein, 45 kD
Distribution:
B cells, follicular dendritic cells
Function:
Regulates B cell activation
Ligand Receptor:
IgE, CD19/CD21/CD81
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Delespesse G, et al. 1992. Immunol. Rev. 125:77. 3. Flores-Romo L, et al. 1993. Science 261:1038.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD23
C57BL/6 mouse splenocytes were stained with biotinylated CD23 (clone B3B4) (filled histogram), or biotinylated rat IgG2a, κ (open histogram), followed by Sav-PE.
FITC anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) FITC (solid line), or rat IgG2a, κ FITC (broken line).
PE anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE (filled histogram), or rat IgG2a, κ PE (open histogram).
Purified anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with purified CD23 (clone B3B4) (filled histogram), or purified rat IgG2a, κ (open histogram), followed by anti-rat IgG FITC.
Alexa Fluor® 488 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Alexa Fluor® 488 (filled histogram), or rat IgG2a, κ Alexa Fluor® 488 (open histogram).
Alexa Fluor® 647 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Alexa Fluor® 647 (filled histogram), or rat IgG2a, κ Alexa Fluor® 647 (open histogram).
PE/Cy7 anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) PE/Cy7 (filled histogram), or rat IgG2a, κ PE/Cy7 (open histogram).
Pacific Blue™ anti-mouse CD23
CD57BL/6 mouse splenocytes were stained with CD23 (clone B3B4) Pacific Blue™ (orange histogram), or rat IgG2a, κ Pacific Blue™(blue histogram).
