The CD11b antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The CD11b antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This CD11b reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats of this clone) include:immunoprecipitation1, 4, in vitro blocking3, 9, 12, depletion2, 8,immunofluorescence microscopy6, 7, 10 and immunohistochemistry of acetone-fixed frozen sections5, 11-13.
Application References:
1. Springer, T., et al., 1978. Eur. J. Immunol. 8:539. (IP) 2. Ault, K., Springer, T., 1981. J. Immunol. 126:359. (Deplete) 3. Springer, T.A., et al., 1982. Immunol. Rev. 68:171. (Block) 4. Ho, MK., Springer, TA., 1983. J Biol Chem. 258:2766. (IP) 5. Flotte, T.J., et al., 1983. Am. J. Pathol. 111:112. (IHC) 6. Noel GJ et al 1990 J Clin Invest. 85:208. (IF) 7. Allen, LA., Aderem, A., 1996. J Exp Med. 184:627 (IF) 8. D'Amico, A., Wu, L., 2003. J. Exp. Med. 198:293. (Deplete) 9. Brickson, SJ., et al., 2003. Appl Physiol. 95:969. (Block) 10. Clatworthy MR, Smith KG. 2004. J Exp Med. 199:717. (IF) 11. Hata, H., et al., 2004. J. Clin. Invest. 114:582. (IHC) 12. Zhang, Y., et al., 2002. J. Immunol. 168: 3088. (IHC) 13. Iwasaki, A., Kelsall, BL., 2001. J Immunol 166:4884 (IHC, FC) 14. Tailleux L 2003 J Exp Med 197:121. (Block, FC) 15. Olver, S., et al., 2006. Cancer Research 66:571. (FC) 16. Tan, SL., et al., 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev, E. D., et al., 2006. J. Immunol. 176:1402. (FC) 18. Dzhagalov, I., et al., 2007. Blood 109:1620. (FC) 19. Fazilleau, N., et al., 2007. Nature Immunol. 8:753. 20. Rasmussen, J.W., et al., 2006. Infect. Immun.74:6590. PubMed 21. Napimoga, MH.,et al., 2008. J. Immunol. 180:609. PubMed 22. Elqaraz-Carmon, V., et al., 2008. J Lipid Res. PubMed 23. Kim DD., et al. 2008. Blood.PubMed 24. Guo, Y., et al., 2008. Blood. 112:480. PubMed 25. Norian, LA., et al., 2009. Cancer Res. 69:3086. (FC) PubMed 26. Baumgartner, C.K., et al., 2010. J. Immunol. 184:573. PubMed 27. Charles, N., et al., 2010. Nat. Med. 16:701. (FC) PubMed
C57BL/6 mouse Bone marrow stained with Pacific Blue™ M1/70
Description:
CD11b is a 170 kD glycoprotein, also known as αM integrin, Mac-1 α subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, NK cells, and some T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The M1/70 antibody can block CD11b-mediated cellular adhesion. This antibody is also cross-reactive with human CD11b.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes, NK cells, some T and B cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
