The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC6-9
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation1, in vitro blocking of cell-cell adhesion2, and immunohistochemistry3-5 of acetone-fixed frozen
Application References:
1. Springer, T., et al., 1978. Eur. J. Immunol. 8:539. 2. Springer, T.A., et al., 1982. Immunol. Rev. 68:171. 3. Flotte, T.J., et al., 1983. Am. J. Pathol. 111:112. 4. Hata, H., et al., 2004. J. Clin. Invest. 114:582. 5. Zhang, Y., et al., 2002. J. Immunol. 168: 3088. 6. Olver, S., et al., 2006. Cancer Research 66:571. 7. Tan, S-L., et al., 2006. J. Immunol. 176:2872. 8. Ponomarev, E. D., et al., 2006. J. Immunol. 176:1402. 9. Dzhagalov, I., et al 2007. Blood 109:1620. 10. Fazilleau, N., et al., 2007. Nature Immunol. 8:753.
C57BL/6 mouse Bone marrow stained with Pacific Blue™ M1/70
Description:
CD11b is a 170 kD glycoprotein, also known as αM integrin, Mac-1 α subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, NK cells, and some T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The M1/70 antibody can block CD11b-mediated cellular adhesion. This antibody is also cross-reactive with human CD11b.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes, NK cells, some T and B cells
