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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry2. The 2B8 antibody does not block c-Kit activity.
Application References:
1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. 2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed 3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed 4. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Pacific Blue™ rat IgG2b isotype control
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Pacific Blue™
CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells.
Other Names:
c-KIT, Stem Cell Factor Receptor (SCFR)
Structure:
Ig superfamily, 145 kD
Distribution:
Hematopoietic stem cells, AML, mast cells
Function:
Growth factor receptor, tyrosine kinase
Ligand Receptor:
Stem Cell Factor (SCF)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells were stained with CD45R/B220 PE and CD117 (clone 2B8) APC (top) or rat IgG2b, κ APC isotype control (bottom).
Biotin anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with B220 APC and biotinylated CD117 (clone 2B8) or rat IgG2b, κ isotype control, followed by Sav-PE.
FITC anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained
with 2B8 PE and CD11b FITC
PE anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained
with 2B8 PE and CD11b FITC
PE/Cy5 anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained
with 2B8 PE and CD11b FITC
Purified anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained
with 2B8 PE and CD11b FITC
PE/Cy7 anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cy7
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Alexa Fluor® 647 rat IgG2a isotype control
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Alexa Fluor® 647
Pacific Blue™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and Pacific Blue™ rat IgG2b isotype control
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 Pacific Blue™
PerCP/Cy5.5 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP/Cy5.5 rat IgG2a isotype control
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 PerCP/Cy5.5
PerCP anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP rat IgG2a isotype control
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 PerCP
APC/Cy7 anti-mouse CD117 (c-kit)
C57BL/6 bone marrow cells stained with B220 (RA3-6B2) PE and 2B8 APC/Cy7
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (clone 2B8) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphoid cell population.
