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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.
Application References:
1. Ge AZ and Butcher EC. 1994. Gene 138:201. 2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC) 3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC) 4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)
CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-ß1, TGF-ß3 and modulates TGF-ß signaling by interacting with TGF-ß receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.
Other Names:
Endoglin
Structure:
Type I integral membrane protein, , homodimer, TGF-ß type III receptor family member
Distribution:
Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotropholast
Function:
Suppress TGF-ß signaling, angiogenesis
Ligand Receptor:
TGF-ß1, TGF-ß3
Antigen References:
1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925. 2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027. 3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584. 4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393. 5. Duff S, et al. 2003. FASEB J. 17:984. 6. Warrington K, et al. 2005. Anticancer Res. 25:185
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD105
BEND.3, mouse endothelial cells stained with MJ7/18 biotin, followed Sav-PE
PE anti-mouse CD105
BEND.3, mouse endothelial cells stained with MJ7/18 PE
PE/Cy7 anti-mouse CD105
BEND.3 mouse endothelial cells stained with MJ7/18 PE/Cy7
Pacific Blue™ anti-mouse CD105
bEND.3 cells stained with MJ7/18 Pacific Blue™
APC anti-mouse CD105
Mouse endothelial cell line bEnd.3 stained with MJ7/18 APC
