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The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections, and in vitro costimulation of T cell activation1,3,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 313512).
Application References:
1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) 2. Yagi J, et al. 2003. J. Immunol. 171:783. (FC) 3. Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) 4. Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
PHA-activated (3 days) human peripheral blood lymphocytes were stained CD278 (clone C398.4A) Pacific Blue™ (filled histogram) or Armenian hamster IgG, κ Pacific Blue™ isotype control (open histogram).
ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.
Other Names:
Inducible COStimulatory molecule, H4, CD278
Structure:
CD28/CTLA-4, 47-57 kD
Distribution:
Activated T cells, subset of thymocytes
Function:
Costimulates T cell activation, proliferation, humoral immune response
Ligand Receptor:
B7h/B7RP-1/GL-50
Antigen References:
1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified C398.4A, followed by biotinylated anti-Armenian hamster IgG and Sav-PE
LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with LEAF™ purified C398.4A, followed by anti-Armenian hamster IgG FITC
Biotin anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with biotinylated C398.4A, followed by Sav-PE
FITC anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A FITC
PE anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A PE
APC anti-human/mouse/rat CD278 (ICOS)
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with C398.4A APC
