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Pacific Blue™ anti-human IgM Antibody
Pacific Blue™ anti-human IgM Antibody
314513 25 µg $140.00     
314514 100 µg $295.00     
Product Details
Clone: MHM-88
Isotype: Mouse IgG1, κ
Isotype Control:Pacific Blue™ Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: Human IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
** Pacific Blue™ is a registered trademark of Molecular Probes, Inc.  Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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Application
Notes:

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Over night cultured human peripheral
Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom)




Compare all formats



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
GeneID: 959
Latest Publications: View the latest IgM articles on HighwirePress.com
UniProt: View information about IgM on UniProt.org
Keywords: Pacific Blue™ anti-human IgM, MHM-88, Pacific Blue™, Immunoglobulin M , Human, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human IgM
    Over night cultured human peripheral

    Over night cultured human peripheral blood lymphocytes stained with purified MHM-88, followed by anti-mouse IgG FITC

  • PE anti-human IgM
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human IgM (clone MHM-88) PE (top) or mouse IgG1, κ PE isotype control (bottom).





  • Biotin anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD19 APC and biotinylated MHM-88, followed by Sav-PE

  • FITC anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHM-88 FITC

  • APC anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHM-88 APC

  • PerCP/Cy5.5 anti-human IgM
    Overnight cultured human peripheral blood

    Overnight cultured human peripheral blood lymphocytes stained with MHM-88 PerCP/Cy5.5

  • Pacific Blue™ anti-human IgM
    Over night cultured human peripheral

    Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom)





  • Brilliant Violet 421™ anti-human IgM
    Isolated human peripheral blood mononuclear

    Isolated human peripheral blood mononuclear cells were stained with CD19 FITC and IgM (clone MHM-88) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphocyte population.





  • APC/Cy7 anti-human IgM
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were cultured overnight and then stained with CD19 PE and anti-human IgM (clone MHM-88) APC/Cy7 (top) or mouse IgG1, κ APC/Cy7 isotype control (bottom).





  • Brilliant Violet 570™ anti-human IgM
    Isolated human peripheral blood mononuclear

    Isolated human peripheral blood mononuclear cells were stained with CD19 FITC and anti-human IgM (clone MHM-88) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom). Data shown was gated on lymphocyte population.





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