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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The MHL-38 antibody reacts with both soluble and membrane human immunoglobulin light chain lambda (λ). It does not react with human immunoglobulin light chain kappa (k) or heavy chain (μ). The MHL-38 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human Ig light chain λ
Over night cultured human peripheral blood lymphocytes stained with purified MHL-38, followed by anti-mouse IgG FITC
FITC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 FITC
CD19+ human B-lymphocytes stained with MHL-38 (anti-λ) FITC and MHK-49 (anti-κ) PE
PE anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 PE
APC anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and MHL-38 APC
Alexa Fluor® 488 anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with MHL-38 Alexa Fluor® 488
Alexa Fluor® 647 anti-human Ig light chain λ
Human peripheral blood lymphoctes stained with MHL-38 Alexa Fluor® 647
Pacific Blue™ anti-human Ig light chain λ
Human peripheral blood lymphocytes stained with CD19 PE and MHL-38 Pacific Blue™ (top) or mIgG2a,κ Pacific Blue™ isotype control (bottom).
