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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2
.
Application References:
1. Trimble L, et al. 1998. Blood 91:585. 2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. 3. Raqib R, et al. 2002. Infect. Immun. 70:3199. 4. Chen H, et al. 2005. J. Immunol. 175:591. 5. Cellerai C, et al. 2010. J. Virol. 84:3868. PubMed
Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors:
Assisted by perforin
Bioactivity/Activities:
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Antigen References:
1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human Granzyme A
Human peripheral blood lymphocytes surface stained with CD8a APC and then intracellularly stained with CB9 FITC
PE anti-human Granzyme A
Human peripheral blood lymphocytes intracellular stained with CB9 PE
Purified anti-human Granzyme A
Human peripheral blood lymphocytes intracellular stained with purified CB9, followed by anti-mouse IgG FITC
Pacific Blue™ anti-human Granzyme A
Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
Alexa Fluor® 700 anti-human Granzyme A
Human peripheral blood lymphocytes surface stained with CD8 FITC, then intracellularly stained with CB9 Alexa Fluor® 700
Alexa Fluor® 488 anti-human Granzyme A
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellularly stained with CB9 Alexa Fluor® 488
Alexa Fluor® 647 anti-human Granzyme A
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellularly stained with CB9 Alexa Fluor® 647
PerCP/Cy5.5 anti-human Granzyme A
Human peripheral blood lymphocytes intracellular stained with CB9 PerCP/Cy5.5
