The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
ICFC
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
Application References:
1. Trimble, L., et al., 1998. Blood 91: 585. 2. Beresford, P., et al., 1997. PNAS 94: 9285. 3. Raqib, R., et al., 2002. Infect. Immun. 70: 3199. 4. Chen, H., et al., 2005. J. Immunol. 175: 591.
Human peripheral blood lymphocytes intracellularly stained with CD8 (RPA-T8) PerCP and CB9 Pacific Blue™
Description:
Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors:
Assisted by perforin
Bioactivity/Activities:
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Antigen References:
1. Brune, J., et al., 1986. Nature 322:268. 2. Fan, Z., et al., 2003. Nature Immunol. 4:145. 3. Fan, Z., et al., 2003. Cell 112:659. 4. Masson, D., et al., 1987. Cell 49:679.
