The CD86 antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
test sizes: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling:
The CD86 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. µg format: For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. test format: For immunofluorescent staining, the suggested use of this reagent is 20 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Western blotting3, and blocking of T cell activation2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 305410).
Application References:
1. Kishimoto, T., et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing, Inc. London. 2. Dieu, M. 1998. J. Exp. Med. 188:373. 3. Esser, M. et al., 2001. J. Virol. 75:6173. 4. Jeannin, P. et al., 1999. J. Immunol. 162:2044. 5. Kapsogeorgou, E. K. et al., 2001. J. Immunol. 166:3107. 6. Geissmann, F.,et al. 2001. Blood 97:1241.
Human peripheral blood monocytes stained with IT2.2 Pacific Blue™
Description:
CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, known as CTLA-4, to deliver an inhibitory signal to T cells. The IT2.2 antibody blocks T cell activation.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
