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Pacific Blue™ anti-human CD64 Antibody
Pacific Blue™ anti-human CD64 Antibody
305017 25 µg $105.00     
305018 100 µg $225.00     

Product Details

Clone: 10.1
Isotype: Mouse IgG1, κ
Isotype Control:Pacific Blue™ Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
VI MA36
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Capuchin Monkey, Squirrel Monkey
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.



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COA:
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Application
References:

1. McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
3. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
4. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717.
5. Holl V, et al. 2004. J. Immunol. 173:6274.
6. Hober D, et al. 2002. J. Gen. Virol. 83:2169.
7. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006
8. Van Tits L, et al.2005. Arteisocler Thromb Vasc Biol. 25:717. PubMed
9. Bruhns P, et al. 2008. Blood PubMed
10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
11. Carter DL, et al. 1999. Cytometry 37:41. (FC)

Human peripheral blood monocytes stained
Human peripheral blood monocytes stained with 10.1 Pacific Blue™


Compare all formats



Description:

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Other Names: FcγRI, FcR I
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand Receptor: IgG receptor
Antigen
References:

1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.

GeneID: 2209
Latest Publications: View the latest CD64 articles on HighwirePress.com
UniProt: View information about CD64 on UniProt.org
Keywords: Pacific Blue™ anti-human CD64, 10.1, Pacific Blue™, FcγRI, FcR I, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Capuchin Monkey, Squirrel Monkey, Immunology, Antibodies
Related Products

DescriptionCloneApplications
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Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human CD64
    Human peripheral blood monocytes stained
with

    Human peripheral blood monocytes stained with 10.1 FITC

  • FITC anti-human CD64
    Human peripheral blood monocytes stained
with

    Human peripheral blood monocytes stained with 10.1 FITC

  • PE anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with 10.1 PE

  • Purified anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with purified 10.1, followed by anti-mouse IgG FITC

  • Alexa Fluor® 488 anti-human CD64
    Human peripheral blood lymphocytes, monocytes,

    Human peripheral blood lymphocytes, monocytes, and granulocytes stained with 10.1 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with10.1 Alexa Fluor® 647

  • APC anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with 10.1 APC

  • LEAF™ Purified anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with purified 10.1, followed by anti-mouse IgG FITC

  • Pacific Blue™ anti-human CD64
    Human peripheral blood monocytes stained

    Human peripheral blood monocytes stained with 10.1 Pacific Blue™

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