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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Western blotting2,3, and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 304812).
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. (WB Block) 3. Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) 4. Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed 5. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed 6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 7. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 9. Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB)
Human peripheral blood lymphocytes stained with DREG-56 Pacific Blue™
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis x (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naive lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
Other Names:
L-selectin, LECAM-1, LAM-1, Leu-8, MEL-14, TQ-1
Structure:
Selectin, single chain glycoprotein, 74-95 kD
Distribution:
Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function:
Leukocyte homing, leukocyte tethering, rolling
Ligand Receptor:
CD34, GlyCAM, MAdCAM-1
Antigen References:
1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 2. Kishimoto T, et al. 1991. Blood 78:805.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 APC
FITC anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 FITC
LEAF™ Purified anti-human CD62L
Human peripheral blood lymphocytes stained with LEAF™ purified DREG-56, followed by anti-mouse IgGs FITC
PE anti-human CD62L
Human peripheral blood lymphocytes stained
with DREG-56 PE
PE/Cy5 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PE/Cy5
Purified anti-human CD62L
Human peripheral blood lymphocytes stained with purified DREG-56, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 APC/Cy7
Alexa Fluor® 488 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG56 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Alexa Fluor® 700
PE/Cy7 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PE/Cy7
PerCP/Cy5.5 anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 PerCP/Cy5.5
Pacific Blue™ anti-human CD62L
Human peripheral blood lymphocytes stained with DREG-56 Pacific Blue™
Brilliant Violet 421™ anti-human CD62L
Human peripheral blood lymphocytes were stained with CD62L (clone DREG-56) Brilliant Violet 421™ (filled histogram) or mouse IgG1 Brilliant Violet 421™ isotype control (open histogram).
