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The CD56 antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
test size: lot-specific; µg size: 0.5 mg/ml
Storage & Handling:
The CD56 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells or 5 µl per 100 µl of whole blood. For µg size, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).
Application References:
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 2. Hanaoka N, et al. 2010. J. Immunol. 185:5732. PubMed
Human peripheral blood lymphocytes stained with MEM-188 Pacific Blue™
CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.
Other Names:
NCAM, Leu-19, NKH1
Structure:
Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution:
NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function:
Adhesion
Ligand Receptor:
Heparin sulfate
Antigen References:
1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 APC
FITC anti-human CD56 (NCAM)
Whole blood lymphocytes stained
with MEM-188 FITC
PE anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 PE
PE/Cy5 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 PE/Cy5
Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with purified MEM-188, followed by anti-mouse IgGs FITC
LEAF™ Purified anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with LEAF™ purified MEM-188, followed by anti-mouse IgG FITC
Alexa Fluor® 488 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 Alexa Fluor® 647
Biotin anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with biotinylated MEM-188, followed by Sav-PE
Pacific Blue™ anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 Pacific Blue™
PE/Cy7 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 PE/Cy7
PerCP/Cy5.5 anti-human CD56 (NCAM)
Human peripheral blood lymphocytes stained with MEM-188 PerCP/Cy5.5
