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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: Western blotting2, and immunohistochemical staining2,5 of acetone-fixed frozen sections.
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. 2. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC) 3. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. 4. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed 5. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ (open histogram).
CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
Other Names:
Leu-1, Ly-1, T1, Tp67
Structure:
Scavenger receptor superfamily, 67 kD
Distribution:
T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
Biotin anti-human CD5
Human peripheral blood lymphocytes were stained with biotinylated CD5 (clone UCHT2) (filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram).
FITC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) FITC (filled histogram) or mouse IgG1, κ FITC (open histogram).
PE anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PE (solid line) or mouse IgG1, κ PE (dashed line).
Purified anti-human CD5
Human peripheral blood lymphocytes were stained with purified CD5 (UCHT2) (solid line) or purified mouse IgG1, κ (dashed line), followed by anti-mouse IgG PE.
Alexa Fluor® 647 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 (open histogram).
PerCP anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PerCP (filled histogram) or mouse IgG1, κ PerCP (open histogram).
PerCP/Cy5.5 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 (open histogram).
PE/Cy7 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PE/Cy7 (red line) or mouse IgG1, κ PE/Cy7 (green line).
Pacific Blue™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ (open histogram).
Brilliant Violet 421™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (clone UCHT2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ (open histogram).
