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The CD45RA antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling:
The CD45RA antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this CD45RA antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 20 µl per million cells in 100 µl volume. For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, and immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4.
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block) 3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC) 4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC) 5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed 6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed 7. Palendira U, et al. 2008. Blood (FC)PubMed 8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed 10. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 11. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed 12. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed 13. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 14. Guereau-de-Arellan M, et al. 2011. Brain. PubMed.
Human peripheral blood lymphocytes stained with HI100 Pacific Blue™
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naive T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
Structure:
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution:
B cells, naïve T cells, monocytes
Function:
Enhances TCR and BCR signaling
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 APC
Biotin anti-human CD45RA
Human peripheral blood lymphocytes stained with biotinylated HI100,followed by Sav-PE
FITC anti-human CD45RA
Human peripheral blood lymphocytes stained
with HI100 FITC
PE anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA (clone HI100) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
PE/Cy5 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 PE/Cy5
Purified anti-human CD45RA
Human peripheral blood platelets stained with purified HI100, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa Fluor® 488
Pacific Blue™ anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Pacific Blue™
Alexa Fluor® 700 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa Fluor® 700
PerCP/Cy5.5 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 PerCP/Cy5.5
PE/Cy7 anti-human CD45RA
Human peripheral blood lymphocytes were stained with HI100 PE/Cy7 (filled histogram) or mouse IgG2b, κ PE/Cy7 isotype control (open histogram).
APC/Cy7 anti-human CD45RA
Human peripheral blood lymphocytes were stained CD45RA (clone HI100) APC/Cy7 (filled histogram) or mouse IgG2b, κ APC/Cy7 isotype control (open histogram).
Brilliant Violet 421™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 570™ (top) or mouse IgG2b, κ Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 605™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 605™.
Brilliant Violet 650™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO FITC and CD45RA (clone HI100) Brilliant Violet 650™.
