The antibody was purified by affinity chromatography, and conjugated with Pacific Blue ™ under optimal conditions. The solution is free of unconjugated Pacific Blue ™
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC -Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
ABCG2 transfected cells stained with 5D3 Pacific Blue™
Description:
CD338, known as ABCG2 (BCRP1), is a multi-drug resistance (MDR) family protein that is a member of ATP binding cassette (ABC) transporters. It is highly expressed on primitive ‘sidepopulation’ (SP) stem cells. This SP phenotype is based on the efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. The SP cells express low or undetectable levels of CD34. In the bone marrow, about 0.05% population of cells displays the low fluorescence and are highly enriched for repopulating cells. The highest levels of BCRP1 mRNA expression have been seen in KDR+ human stem cells. The expression of ABCG2 appears to be highly conserved as it has been identified in various species.
Other Names:
ABCG2, CDw338, Bcrp1, MXR
Distribution:
primitive ‘sidepopulation’ (SP) stem cells, KDR+ human stem cells
Antigen References:
1. Kim, M., et al., 2002. Clin. Cancer Res. 8:228. 2. Scharenber, C.W., et al., 2002. Blood. 99:50712. 3.Goodell, M.A., et al., 1997. Nat. Med. 3:133745. 4. Bunting, K.D., 2002. Stem Cells. 20:11.
