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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections, and blocking of platelet aggregation3. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303108).
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. 2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) 3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) 4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed 5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed 6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Human peripheral blood monocytes were stained with CD31 (clone WM59) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation. Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.
Other Names:
PECAM-1, EndoCAM
Structure:
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
PE anti-human CD31
Human peripheral granulocytes were stained with CD31 (clone WM59) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Purified anti-human CD31
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with purified WM59, then
detected with anti-mouse IgG FITC
LEAF™ Purified anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with LEAF™ purified CD31 (clone WM59), followed by anti-mouse IgG-FITC.
Alexa Fluor® 488 anti-human CD31
Human peripheral blood granulocytes were stained with CD31 (clone WM59) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
Alexa Fluor® 647 anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) Alexa Fluor® 647.
Pacific Blue™ anti-human CD31
Human peripheral blood monocytes were stained with CD31 (clone WM59) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
APC anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) APC.
PE/Cy7 anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) PE/Cy7.
APC/Cy7 anti-human CD31
Human peripheral blood lymphocytes, monocytes and granulocytes were stained with CD31 (clone WM59) APC/Cy7 (filled histogram) or mouse IgG1, κ APC/Cy7 isotype control (open histogram).
