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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation4-7 of T lymphocytes. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300314).
Application References:
1.Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ) 5. Rivollier A, et al. 2004. Blood 104:4029. (Activ) 6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ) 7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Human peripheral blood lymphocytes stained with HIT3a Pacific Blue™
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The HIT3a antibody is able to stimulate T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a APC
Biotin anti-human CD3
Human peripheral blood lymphocytes stained with biotinylated HIT3a, followed by Sav-PE
FITC anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a FITC
LEAF™ Purified anti-human CD3
Human peripheral blood lymphocytes stained with LEAFTM purified HIT3a and detected with anti-mouse IgGs FITC
PE anti-human CD3
Human peripheral blood lymphocytes stained
with HIT3a PE
PE/Cy5 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a PE/Cy5
Purified anti-human CD3
Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC
APC/Cy7 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a APC/Cy7
PE/Cy7 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a PE/Cy7
Alexa Fluor® 488 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 700
PerCP/Cy5.5 anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a PerCP/Cy5.5
Pacific Blue™ anti-human CD3
Human peripheral blood lymphocytes stained with HIT3a Pacific Blue™
