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Pacific Blue™ anti-human CD3 Antibody
Pacific Blue™ anti-human CD3 Antibody
300418 25 µg $95.00 Qty:
    
300417 100 µg $225.00 Qty:
    
300431 100 tests $240.00 Qty:
    
300442 500 tests $1,000.00 Qty:
    

Product Details

 
Clone: UCHT1 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Pacific Blue™ Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
III 471
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration: test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation
Laser:
Violet Laser (405 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library
Pacific Blue™ anti-human CD3 Antibody UCHT1 Image 1
Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™


Compare all formats

See Pacific Blue™ spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

GeneID: 915
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: Pacific Blue™ anti-human CD3, UCHT1, Pacific Blue™, PB, T3, CD3ε, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD3UCHT1FC, IF
Alexa Fluor® 594 anti-human CD3UCHT1IF
Alexa Fluor® 647 anti-human CD3UCHT1FC, IF
Alexa Fluor® 700 anti-human CD3UCHT1FC
APC anti-human CD3UCHT1FC
APC/Cy7 anti-human CD3UCHT1FC
Biotin anti-human CD3UCHT1FC
Brilliant Violet 421™ anti-human CD3UCHT1FC, IF
Brilliant Violet 510™ anti-human CD3UCHT1FC
Brilliant Violet 570™ anti-human CD3UCHT1FC
Brilliant Violet 605™ anti-human CD3UCHT1FC
FITC anti-human CD3UCHT1FC
LEAF™ Purified anti-human CD3UCHT1FC, Activ, IHC, IP, WB, CyTOF®
PE anti-human CD3UCHT1FC
PE/Cy5 anti-human CD3UCHT1FC
PE/Cy7 anti-human CD3UCHT1FC
PE/Dazzle™ 594 anti-human CD3UCHT1FC
PerCP anti-human CD3UCHT1FC
PerCP/Cy5.5 anti-human CD3UCHT1FC
Purified anti-human CD3UCHT1FC, IHC, IP, WB, CyTOF®
Purified anti-human CD3 (MaxPar® Ready)UCHT1FC, CyTOF®
Ultra-LEAF™ Purified anti-human CD3UCHT1FC, Activ, IHC, IP, WB, CyTOF®


Related Pathways

Pathway
CD28 Signaling in T-Helper Cell
CD4 and CD8 T-Cell Lineage
ERK Signaling
Gamma-Delta T cells
Immunologic Networks 2011
Innate Immunity
MDSC-induced Immune Suppression
NFAT Signaling and Lymphocyte Interactions
TH1 Pathway (Cellular Immune Response)
Th17 Biology
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Non-Human Primate Immunology > CD3
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD3 (T3)
Immunology > Human Immunology > Th17 > CD3 > UCHT1
UCHT1
Immunology > Human Immunology > Costimulatory Molecules > CD3 > UCHT1
Immunology > Human Immunology > CD and Related Molecules > CD3 (T3) > UCHT1
Immunology > Human Immunology > Innate Immunity > CD3 > UCHT1
CD3 > UCHT1
UCHT1
Immunology > Human Immunology > Stem Cells > CD3 (T3) > UCHT1
Immunology > Human Immunology > NK/NKT > CD3 > UCHT1
Immunology > Human Immunology > Th1/Th2 > CD3 > UCHT1

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 APC

  • Biotin anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated UCHT1 and then detected with Sav-PE

  • FITC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 FITC

  • LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).





  • PE anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE

  • PE/Cy5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE/Cy5

  • Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC

  • Alexa Fluor® 647 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 647.

    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD19 (clone HIB19) Brilliant Violet 421™ (blue) and 5µg/ml CD3 (clone UCHT1) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Alexa Fluor® 488 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 488®

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.

  • Pacific Blue™ anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™

  • PE/Cy7 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE/Cy7 and overlayed with isotype control

  • Alexa Fluor® 700 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700

  • APC/Cy7 anti-human CD3
    Human peripheral lymphocytes stained with

    Human peripheral lymphocytes stained with UCHT1 APC/Cy7

  • PerCP anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PerCP

  • PerCP/Cy5.5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PerCP/Cy5.5

  • Brilliant Violet 421™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone UCHT1) Brilliant Violet 421™ (blue) and 5 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Brilliant Violet 570™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 APC and CD3 (clone UCHT1) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • Ultra-LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).





  • Purified anti-human CD3 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 170Er-anti-CD3 (UCHT1). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-human CD3
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (yellow), 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green), and 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 594 (red) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

  • PE/Dazzle™ 594 anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

  • Brilliant Violet 510™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).

  • Brilliant Violet 605™ anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD3 (clone UCHT1) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).

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