Antibodies
      
 
Product Search  
 
Pacific Blue™ anti-human CD3 Antibody
Cat. # Size Price  

300418 25 µg $95.00
    
Reviews (1)
300417 100 µg $225.00
    
Reviews (1)
300431 100 tests $240.00
    
Reviews (1)
300442 500 tests $1,000.00
    
Reviews (1)
Product Details

Clone: UCHT1 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Pacific Blue™ Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
III 471
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration: test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation
Laser:
Violet Laser (405 nm)
Application
Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, and Western blotting9. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 300414). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300438) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™


Compare all formats

See Pacific Blue™ spectral data





 


Antigen Details

Description: CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
GeneID: 915
UniProt: View information about CD3 on UniProt.org
Keywords: Pacific Blue™ anti-human CD3, UCHT1, Pacific Blue™, PB, T3, CD3ε, Human, Cross-Reactivity: Chimpanzee, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
Request Bulk Quote
Request Custom Conjugation
Email Technical Support
Write a Review
View All CD3 Reagents
Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Rating

   Average Rating
Related Pages

Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 APC

  • Biotin anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated UCHT1 and then detected with Sav-PE

  • FITC anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 FITC

  • LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).





  • PE anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE

  • PE/Cy5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE/Cy5

  • Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC

  • Alexa Fluor® 647 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 647.

    Human peripheral mononuclear cells were

    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD19 (clone HIB19) Brilliant Violet 421™ (blue) and 5µg/ml CD3 (clone UCHT1) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Alexa Fluor® 488 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 488®

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.

  • Pacific Blue™ anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Pacific Blue™

  • PE/Cy7 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PE/Cy7 and overlayed with isotype control

  • Alexa Fluor® 700 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700

  • APC/Cy7 anti-human CD3
    Human peripheral lymphocytes stained with

    Human peripheral lymphocytes stained with UCHT1 APC/Cy7

  • PerCP anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PerCP

  • PerCP/Cy5.5 anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with UCHT1 PerCP/Cy5.5

  • Brilliant Violet 421™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone UCHT1) Brilliant Violet 421™ (blue) and 5 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Brilliant Violet 570™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 APC and CD3 (clone UCHT1) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).





  • Ultra-LEAF™ Purified anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).





  • Purified anti-human CD3 (MaxPar® Ready)
    Human PBMCs stained with 154Sm-anti-CD45

    Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 170Er-anti-CD3 (UCHT1). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-human CD3
    Human peripheral blood mononuclear cells

    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (yellow), 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 488 (green), and 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 594 (red) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Alexa Flour® 594 (filled histogram) or mouse IgG1, κ Alexa Flour® 594 isotype control (open histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with the Yellow-Green Laser (561 nm).

  • PE/Dazzle™ 594 anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

  • Brilliant Violet 510™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).

  • Brilliant Violet 605™ anti-human CD3
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD3 (clone UCHT1) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).

  • Brilliant Violet 711™ anti-human CD3
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).

x
x
*Your Rating:
*Your Comment:
*Name (first, last):
*Affiliation:
*Email:
*Required fields
Note: Submission of a quick review does not qualify for our promotional $25 Amazon gift card. A full review submission is required for gift card eligibility.

Learn more…
9727 Pacific Heights Blvd.
San Diego, CA 92121

Tel: 858.768.5800
Company
Careers
Promotions
Web Tools
Areas of Biology
Pathways
Products
Literature
Custom Products/Services
Custom Panel Construction
Technical Support
Support Resources
Ordering
Contact
Distributors
Sitemap
Connect with us:

Facebook Twitter Pinterest Instagram LinkedIn YouTube Tumblr Blog Podcast
The LEGENDeers Pandora Google+ Wikipedia RSS Feed Arcade Words With Elements Satisfaction Survey
Privacy Policy | Terms
World-Class Quality | Superior Customer Support | Outstanding Value Copyright © 2014 BioLegend, Inc. All rights reserved.
Product Search