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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA) 2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA) 3. Velu V, et al. 2007. J. Virol. 81:5819. 4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed 5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed 6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA) 7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed 8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed 9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed 10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed 11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Pacific Blue™ and CD3 (clone UCHT1) FITC.
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ (open histogram).
The program death 1 (PD-1) receptor CD279 is a 55 kD member of the immunoglobulin superfamily. CD279/PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279/PD-1 is expressed predominantly on activated T cells, B cells and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279/PD-1 and are members of the B7 gene family. Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279:PD-ligands results in inhibition of T cell proliferation and cytokine secretion.
Other Names:
PD-1
Structure:
Immunoglobulin superfamily
Distribution:
Transiently expressed on CD4- CD8- thymocytes; stimulated thymocytes and splenic T and B lymphocytes; activated myeloid cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Brilliant Violet 421™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (clone EH12.2H7) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Purified anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with purified CD279 (clone EH12.2H7) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
FITC anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) FITC (filled histogram) or mouse IgG1, κ FITC (open histogram).
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) FITC and CD3 (clone UCHT1) PerCP/Cy5.5.
PE anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE (filled histogram) or mouse IgG1, κ PE (open histogram).
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE and CD3 (clone UCHT1) PerCP/Cy5.5.
APC anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC (filled histogram) or mouse IgG1, κ APC (open histogram).
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC and CD3 (clone UCHT1) PerCP/Cy5.5.
Alexa Fluor® 647 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Alexa Fluor® 647 and CD3 (clone UCHT1) PerCP/Cy5.5.
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 (open histogram).
PerCP/Cy5.5 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PerCP/Cy5.5 (blue histogram) or mouse IgG1, κ PerCP/Cy5.5 (red histogram).
APC/Cy7 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes stained with CD279 (clone EH12.2H7) APC/Cy7 (filled histogram) or mouse IgG1, APC/Cy7 (open histogram).
Pacific Blue™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Pacific Blue™ and CD3 (clone UCHT1) FITC.
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ (open histogram).
PE/Cy7 anti-human CD279 (PD-1)
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 (open histogram).
Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE/Cy7 and CD3 (clone UCHT1) FITC.
Brilliant Violet 605™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (clone EH12.2H7) Brilliant Violet 605™.
