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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: blocking of T cell activation, and partical blocking of B cell costimulation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 309212).
Application References:
1. Schlossman S, et al. Eds.1995. Leucocyte Typing V Oxford University Press. New York. 2. Hughes CCW, et al. 1996. J. Biol. Chem. 271:5369.
Human peripheral blood lymphocytes stained with TS1/8 Pacific Blue™
CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.
Other Names:
LFA-2, T11, SRBC-R
Structure:
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution:
T cells, NK cells, thymocytes, and thymic B cell subsets
Function:
T cell activation, adhesion
Ligand Receptor:
CD58 (LFA-3), CD48, CD59, CD15
Antigen References:
1. Bell G, et al. 1995. J. Immunol. 155:2805. 2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD2
Human peripheral blood lymphocytes stained with biotinylated TS1/8 and detected with Sav-PE
FITC anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 FITC
LEAF™ Purified anti-human CD2
Human peripheral blood lymphocytes stained with LEAFTM purified TS1/8 and detected with anti-mouse IgGs FITC
PE anti-human CD2
Whole blood lymphocytes stained
with TS1/8 PE
PE/Cy5 anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 PE/Cy5
Purified anti-human CD2
Human peripheral blood lymphocytes stained with purified TS1/8 and detected with anti-mouse IgGs FITC
Pacific Blue™ anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 Pacific Blue™
PE/Cy7 anti-human CD2
Human peripheral blood lymphocytes stained with TS1/8 PE/Cy7
Brilliant Violet 421™ anti-human CD2
Human peripheral blood lymphocytes stained with (clone TS1/8) Brilliant Violet 421™ (filled histogram) or mouse IgG1 Brilliant Violet 421™ isotype control (open histogram).
