The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - This application has been quality tested
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Application Notes:
Immunocytochemical staining1
Application References:
1. M. del Salamone, C., et al. 2001. J. Leukoc. Biol. 70: 567 2. de Fraissinette, A., et al. 1988. Exp. Hematol. 16: 764
Human T lymphoblastic leukemia cell line, Molt-4, stained with L161 Pacific Blue™
Description:
CD1c, known as R7 or M241, is a 43 kD member of the five CD1 antigens (CD1a-e) in human. The CD1 molecules are type I glycoprotein with the structural similarities to MHC class I and is non-covalently associated with β2-microglobulin, belong to Ig superfamily. CD1c is expressed on cortical thymocytes, Langerhans cells, dendritic cells, and a subset of B cells. It has been reported that CD1c is also expressed on mature T cells with tightly regulated manner. The function of CD1c is involved in antigen-presentation of glycolipids. It may also act in T cells as an immune regulatory molecule.
Other Names:
R7, M241
Structure:
B cell subset, cortical thymocytes, dendritic cells, and Langerhans cells
Distribution:
Present lipid antigen to CD1c-restricted T cells
Function:
CD1c-restricted TCR
Antigen References:
1. Fainboim, LM. and del C. Salamone. 2002. J. Biol. Regul. Homeost. Agents 16: 125 2. M. del Salamone, C., et al. 2001. J. Leukoc. Biol. 70: 567 3. Zola, H., et al. Eds. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. P42
