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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CCR7 is a chemokine receptor that binds CCL19 and CCL21 (1). CCR7 and its ligands link innate and adaptive immunity through their effects on interactions between T cells and dendritic cells. Naive T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent (2). In addition, during immunological surveillance, B lymphocytes re-circulate between B-cell-rich compartments (follicles or B zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated to lymph node metastasis.
Other Names:
CCR7, BLR2, CDw197, EBI1, CD197, CMKBR7
Structure:
Chemokine receptor, G protein-coupled receptors (GPCR) seven transmembrane receptor
Distribution:
T, B, NK, and dendritic cells
Function:
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs and the migration of dendritic cells into afferent lymphatic vessels
Ligand Receptor:
CCL19 and CCL21
Antigen References:
1) Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2) Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3) Reif K, et al. 2002. Nature 416:94. 4) Nakata B, et al. 2008. Oncology 74:69.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 488 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) APC and TG8/CCR7 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and TG8/CCR7 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD197 (CCR7)
Human peripheral blood lymphocytes stained with CD3 (HIT3a) PE and TG8/CCR7 PerCP/Cy5.5
APC anti-human CD197 (CCR7)
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE and TG8/CCR7 APC
Pacific Blue™ anti-human CD197 (CCR7)
Human peripheral blood lymphocytes stained with CD3 FITC and TG8/CCR7 Pacific Blue™ (top) or mIgG2a,k isotype control Pacific Blue™ (bottom)
