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The CD19 antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling:
The CD19 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this CD19 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume. For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and blocking of B cell proliferation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 302214).
Application References:
1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Bradbury L, et al. 1993. J. Immunol. 151:2915. 4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed 5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed 6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
Other Names:
B4
Structure:
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution:
B lineage (except plasma cells), follicular dendritic cells
Function:
B cell activation and differentiation
Ligand Receptor:
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Antigen References:
1. Tedder T, et al. 1994. Immunol. Today 15:437. 2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 APC
Biotin anti-human CD19
Human peripheral blood lymphocytes stained with biotinylated HIB19, followed by Sav-PE
FITC anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 FITC
LEAF™ Purified anti-human CD19
Human peripheral blood lymphocytes stained with LEAF™ purified HIB19, followed by anti-mouse IgGs FITC
PE anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PE
PE/Cy5 anti-human CD19
Human peripheral blood lymphocytes stained
with HIB19 PE/Cy5
Purified anti-human CD19
Human peripheral blood lymphocytes stained with purified HIB19, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 APC/Cy7
PE/Cy7 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PE/Cy7
Alexa Fluor® 488 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa Flour® 488
Alexa Fluor® 647 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa Flour® 647
Pacific Blue™ anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
Alexa Fluor® 700 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa Fluor® 700
PerCP anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PerCP
PerCP/Cy5.5 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 650™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 650™.
