The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PE/Cy5 and TG1/CXCR3 Pacific Blue™
Description:
CXCR3 highly expressed by T lymphocytes (Th1),natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 ligands require IFN-γ for their expression and are localized to sites of inflammation; therefore, CXCR3 is important for effector lymphocyte recruitment into inflamed tissue such as chronically inflamed human liver, Chrohns disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.
Other Names:
G protein-coupled receptor 9, GRP9, CD183 antigen, CD183, CXCR3
Structure:
CXC-chemokine receptor, G protein-coupled receptor, 7 transmembrane receptor
Distribution:
IL-2 activated T cells, Th1, natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells
Function:
CXCR3 is important for effector lymphocyte recruitment into inflamed tissues
Ligand Receptor:
CXCR3 ligands are the chemokines CXCL9, CXCL10 and CXCL11
Interaction:
CXCR3 promotes adhesion of effector T cells to endothelium and drives transendothelial migration
Antigen References:
1. Loetscher M, et al., 1996. J Exp Med. 184:963-969. 2. Cole KE, et al., 1998. J Exp Med 187:2009-2021. 3. Aksoy MO, et al., 2006. Am J Physiol Lung Cell Mol Physiol 290: L909-L918. 4. Curbishley SM, et al,. 2005. Am J Pathol. 167:887-899. 5. Turner JE, et al ., 2007. Min Rev Med Chem 7:1089-1096. 6. Wenzel J, et al., 2008. J Invest Dermatol 128:67-78.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
