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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
test sizes: lot-specific; µg size: 0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume. For µg size, the suggested use of this reagent for immunofluorescent staining is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.
Other Names:
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with purified W6D3, followed by anti-mouse IgG FITC
FITC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 FITC
PE anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE
APC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 APC
Alexa Fluor® 488 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa Fluor® 647
PE/Cy5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE/Cy5
Biotin anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with biotinylated W6D3, followed by Sav-PE
PerCP anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PerCP
PerCP/Cy5.5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PerCP/Cy5.5
Pacific Blue™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Pacific Blue™.
