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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and immunofluorescence microscopy.
Application References:
1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks S, et al. 1990. Biochem. J. 268:275. 2. Wright S, et al. 1990. Science 249:1434.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD14
Human peripheral blood monocytes stained with purified HCD14, followed by anti-mouse IgG FITC
FITC anti-human CD14
Human peripheral blood monocytes stained with HCD14 FITC
PE anti-human CD14
Human peripheral blood monocytes stained with HCD14 PE
APC anti-human CD14
Human peripheral blood monocytes stained with HCD14 APC
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes stained with HCD14 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes stained with HCD14 alexa Fluor® 647
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes stained with HCD14 Alexa Flour® 700
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
PE/Cy7 anti-human CD14
Human peripheral blood monocytes stained with HCD14 PE/Cy7
APC/Cy7 anti-human CD14
Human peripheral blood monocytes stained with HCD14 APC/Cy7
PerCP/Cy5.5 anti-human CD14
Human peripheral blood monocytes stained with HCD14 PerCP/Cy5.5
Biotin anti-human CD14
Human peripheral blood monocytes stained with biotinylated HCD14 and then detected with Sav-PE
