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The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration:
test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells or 5 µl per 100 µl of whole blood. For µg sizes, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue™ is a registered trademark of Molecular Probes, Inc. Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301810).
Application References:
1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block) 5. Williams KC, et al. 2001. J. Exp. Med. 193:905. (IF) 6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed 7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed 8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood monocytes stained with M5E2 Pacific Blue™
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks S, et al. 1990. Biochem. J. 268:275. 2. Wright S, et al. 1990. Science 249:1434.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD14
Human peripheral blood monocytes stained
with M5E2 APC
FITC anti-human CD14
Human peripheral blood monocytes stained with M5E2 FITC
LEAF™ Purified anti-human CD14
Human peripheral blood monocytes stained with LEAF™ purified M5E2, followed by anti-mouse IgG FITC
PE anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE
Purified anti-human CD14
Human peripheral blood monocytes stained
with M5E2 APC
PE/Cy7 anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE/Cy7
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Fluor® 647
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes stained with M5E2 Pacific Blue™
APC/Cy7 anti-human CD14
Human peripheral blood moncytes stained with M5E2 APC/Cy7
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Fluor® 700
PerCP/Cy5.5 anti-human CD14
Human peripheral blood monocytes stained with M5E2 PerCP/Cy5.5
Biotin anti-human CD14
Human peripheral blood monocytes stained with biotinylated M5E2, followed by Sav-PE
Brilliant Violet 421™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clone M5E2) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clone M5E2) Brilliant Violet 570™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 570™ isotype control (open histogram).
Brilliant Violet 605™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clone M5E2) Brilliant Violet 605™.
